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atg5 d5f5u rabbit mab  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc atg5 d5f5u rabbit mab
    Atg5 D5f5u Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1281 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+atg5/Atg5+Rabbit+mAb/pm41857115-129-39-55
    Average 96 stars, based on 1281 article reviews
    atg5 d5f5u rabbit mab - by Bioz Stars, 2026-10
    96/100 stars

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    other:

    Article Title: Singapore grouper iridovirus utilizes Rab1 to facilitate viral trafficking and evade the immune response by targeting the STING-IRF3 pathway
    Article Snippet: The antibodies, including rabbit anti-GFP (catalog no. AB290), rabbit anti-Rab1 (catalog no. AB302545 ), rabbit anti-p62 (catalog no. AB109012 ), and rabbit anti-β-tubulin (catalog no. AB15568) were obtained from Abcam (Cambridge, UK); the rabbit anti-LC3A/B (catalog no. 12741), rabbit anti-HA (catalog no. 3724), rabbit anti-ATG5 (catalog no. 12994) and mouse anti-HA (catalog no. 2367) were obtained from Cell Signaling Technology (Danvers, MA, USA); and the mouse anti-Flag (catalog no. F1804) was purchased from Sigma-Aldrich (St Louis, MI, USA).

    Article Title: Autophagy in lymphatic endothelial cells promotes lung metastasis
    Article Snippet: Antibodies used were goat anti-LYVE1 (R&D systems, AF2125), rabbit anti-ATG5 (12994S, CST), rabbit anti-LC3B (3868S, CST), Vegfr3 (1:100, 14-5988-82, Thermo Fisher Scientific), Rat anti-B220 (1:100, 103204, Biolegend) overnight at 4°C and corresponding secondary antibodies were added for 1 h at 4°C.

    Article Title: p53 Drives Lung Cancer Regression through a TSC2/TFEB-dependent Senescence Program
    Article Snippet: Membranes were blocked with 5% milk in TBS-Tween and probed with the following primary antibodies: rabbit anti-p53 (Leica Biosystems, cat. #NCL-L-p53-CM5p, RRID: AB_2895247; 1:1,000), rat anti-LAMP1 (DSHB, cat. #1d4b, RRID:AB_2134500; 1:100), rabbit anti-TFEB (Bethyl Laboratories, cat. #A303-673A, RRID: AB_11204751; 1:1,000), rabbit anti-LC3II (Cell Signaling Technology, cat. #3868, RRID: AB_2137707; 1:1,000), mouse anti-GAPDH (Biosynth, cat. #10R-G109A; 1:20,000), rabbit anti-ATG5 (Cell Signaling Technology, cat. #12994, RRID: AB_2630393; 1:1,000), rabbit anti-p62 (Enzo Life Sciences, cat. #BML-PW9860, RRID: AB_2196009; 1:1,000), rabbit anti–4E-BP1 (Cell Signaling Technology, cat. #9644, RRID: AB_2097841; 1:1,000), rabbit anti–phospho-4E-BP1 (Thr70; Cell Signaling Technology, cat. #9455, RRID: AB_330949; 1:1,000), rabbit anti-p70 S6 kinase (Cell Signaling Technology, cat. #2708, RRID: AB_390722; 1:1,000), rabbit anti–phospho-p70 S6 kinase (Thr389; Cell Signaling Technology, cat. #9234, RRID: AB_2269803; 1:1,000), and rabbit anti-TSC2 (Cell Signaling Technology, cat. #4308, RRID: AB_10547134; 1:1,000).

    Article Title: Senecavirus a VP2 protein orchestrates PRDX1 degradation through dual autophagy pathways: macroautophagy and chaperone-mediated autophagy
    Article Snippet: The following antibodies were obtained commercially: rabbit anti-GFP (HuaAn, ET1602), mouse anti-ACTB (Sigma-Aldrich, A5441), rabbit anti-MYC (Merck, 05–419), mouse anti-HA (Sigma-Aldrich, H3663), rabbit anti-Flag (HuaAn, 0912–1), rabbit anti-LAMP2A (Abcam, ab18528), rabbit anti-TOMM20 (Proteintech, 11,802–1-AP), rabbit anti-TIMM23 (Proteintech, 11,123–1-AP), rabbit anti-SQSTM1 (Sigma-Aldrich, SAB5700054), rabbit anti-LC3B (Sigma-Aldrich, L7543), rabbit anti-PRDX1 (ABclonal, A4956), rabbit anti-ATG5 (Cell Signaling Technology, 2630), rabbit anti-HSPA8/HSC70 (ABclonal, A0415), rabbit anti-VDAC1 (ABclonal, A19707), mouse anti-MTOR (Proteintech, 66,888–1-Ig), mouse anti-p-MTOR (Proteintech, 67,778–1-Ig), rabbit anti-PIK3R2/PI3K (ABclonal, A17433), rabbit anti-p-PIK3R2/PI3K (Cell Signaling Technology, 17,366), rabbit anti-AKT (Cell Signaling Technology, 4685), rabbit anti-p-AKT (Cell Signaling Technology, 4075), horseradish peroxidase (HRP)-conjugated anti-rabbit (Sigma-Aldrich, A0545) and anti-mouse (Sigma-Aldrich, A9044) secondary antibodies, and Alexa Fluor 647-labeled goat anti-rabbit IgG (Invitrogen, A21245).

    Western Blot:

    Article Title: Targeting Oxalate Production by Combining Enzyme Inhibition and Proteolysis Activation: A Novel Therapeutic Approach for Primary Hyperoxaluria Type 1
    Article Snippet: Protein concentration was determined using the Quick Start Bradford Dye Reagent (Bio-Rad, 5000205). .. Western blotting was performed using the following primary antibodies: rabbit anti-AGXT (Sigma, HPA035370, 1:1000), rabbit anti-LDHA (Cell Signaling Technology, 2012, 1:1000), rabbit anti-HAO1 (Abcam, 194790, 1:1000), mouse anti- β -actin (Cell Signaling Technology, 3700S, 1:1000), mouse anti-GAPDH (Santa Cruz Biotechnology, sc365062, 1:5000) and rabbit anti-ATG5 (Cell Signaling Technology, 12994S, 1:1000). .. Fluorescent secondary antibodies—donkey antirabbit (Li-Cor, 926–68073, 1:20,000) and donkey antimouse (Li-Cor, 926–32212, 1:20,000)—were used for detection with a Li-Cor Odyssey XF Imager.

    Article Title: Targeting Oxalate Production by Combining Enzyme Inhibition and Proteolysis Activation: A Novel Therapeutic Approach for Primary Hyperoxaluria Type 1.
    Article Snippet: XXXX, XXX, XXX−XXX V Protein concentration was determined using the Quick Start Bradford Dye Reagent (Bio-Rad, 5000205). .. Western blotting was performed using the following primary antibodies: rabbit anti-AGXT (Sigma, HPA035370, 1:1000), rabbit anti-LDHA (Cell Signaling Technology, 2012, 1:1000), rabbit anti-HAO1 (Abcam, 194790, 1:1000), mouse anti-β-actin (Cell Signaling Technology, 3700S, 1:1000), mouse antiGAPDH (Santa Cruz Biotechnology, sc365062, 1:5000) and rabbit anti-ATG5 (Cell Signaling Technology, 12994S, 1:1000). .. Fluorescent secondary antibodies�donkey antirabbit (Li-Cor, 926−68073, 1:20,000) and donkey antimouse (Li-Cor, 926−32212, 1:20,000)� were used for detection with a Li-Cor Odyssey XF Imager.

    Labeling:

    Article Title: Senecavirus a VP2 protein orchestrates PRDX1 degradation through dual autophagy pathways: macroautophagy and chaperone-mediated autophagy.
    Article Snippet: Co-adaptation between viruses and autophagy has equipped viruses with diverse strategies to regulate host redox homeostasis, thereby facilitating viral replication.. However, the mechanisms by which viruses manipulate PRDX1 (peroxiredoxin 1), a key antioxidative enzyme, via autophagy remain poorly understood.. Here, we demonstrate that infection by Senecavirus A (SVA), an emerging picornavirus, induces PRDX1 degradation, and that PRDX1 negatively regulates viral replication.

    Article Title: CTCF/STK38 axis drives tumor progression via the Wnt/β-catenin and Hippo pathways.
    Article Snippet: .. The antibodies used in this study included rabbit anti-STK38 (#11105-1-AP; 1:500; Proteintech, Wuhan, China), rabbit anti-Flag labeled (ab88064; 1:1000; CST, Danvers, USA), rabbit anti-LEF1 (#2230; 1:1000; CST), rabbit antiYAP/TAZ (#8418; 1:1000; CST), rabbit anti-ATG5 (#PTM-5868; 1:1000; PTMBIO, Hangzhou, China), rabbit anti-ATG7 (#PTM-6267; 1:1000; PTMBIO), rabbit anti-BECN1 (#3495; 1:1000; CST), rabbit anti-LC3B (#PTM-6384; 1:1000; PTMBIO), rabbit anti-GAPDH (#2118; 1:1000; CST), and the corresponding secondary antibodies goat anti-rabbit (#S004F; 1:5000; Beijing TDY Biotech, Beijing, China) and goat anti-mouse (#S001F; 1:5000; Beijing TDY Biotech). .. Protein bands were detected using an enhanced chemiluminescence (ECL) reagent kit (Millipore).



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